Survival of Staphylococcus aureus ST398 in the Human Nose after Artificial Inoculation
PLoS ONE , Volume 7 - Issue 11
There is evidence that MRSA ST398 of animal origin is only capable of temporarily occupying the human nose, and it is therefore, often considered a poor human colonizer. We inoculated 16 healthy human volunteers with a mixture of the human MSSA strain 1036 (ST931, CC8) and the bovine MSSA strain 5062 (ST398, CC398), 7 weeks after a treatment with mupirocin and chlorhexidine-containing soap. Bacterial survival was studied by follow-up cultures over 21 days. The human strain 1036 was eliminated faster (median 14 days; range 2-21 days) than the bovine strain 5062 (median 21 days; range 7-21 days) but this difference was not significant (p = 0.065). The bacterial loads were significantly higher for the bovine strain on day 7 and day 21. 4/14 volunteers (28.6%) showed elimination of both strains within 21 days. Of the 10 remaining volunteers, 5 showed no differences in bacterial counts between both strains, and in the other 5 the ST398 strain far outnumbered the human S. aureus strain. Within the 21 days of follow-up, neither human strain 1036 nor bovine strain 5062 appeared to acquire or lose any mobile genetic elements. In conclusion, S. aureus ST398 strain 5062 is capable of adequately competing for a niche with a human strain and survives in the human nose for at least 21 days.
|This work was funded by the European Commission 7th Framework Programme; grant id fp7/223050 - Preventing community and nosocomial spread and infection with MRSA ST 398 - instruments for accelerated control and integrated risk management of antimicrobial resistance (PILGRIM)|
|Organisation||Department of Medical Microbiology and Infectious Diseases|
Slingerland, B.C.G.C, Tavakol, M, McCarthy, A.J, Lindsay, J.A, Snijders, S.V, Wagenaar, J.A, … van Wamel, W.J.B. (2012). Survival of Staphylococcus aureus ST398 in the Human Nose after Artificial Inoculation. PLoS ONE, 7(11). doi:10.1371/journal.pone.0048896