2014-02-01
Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the middle East respiratory syndrome coronavirus
Publication
Publication
Journal of Virology , Volume 88 - Issue 3 p. 1834- 1838
Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection.
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| doi.org/10.1128/JVI.02935-13, hdl.handle.net/1765/68010 | |
| Journal of Virology | |
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| Organisation | Department of Virology |
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Stalin Raj, V. S., Smits, S., Provacia, L., van den Brand, J., Wiersma, L., Ouwendijk, W., Bestebroer, T., Spronken, M., van Amerongen, G., Rottier, P., Fouchier, R., Bosch, B. J., Osterhaus, A.& Haagmans, B. (2014). Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the middle East respiratory syndrome coronavirus. Journal of Virology, 88(3), 1834–1838.https://doi.org/10.1128/JVI.02935-13 |
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