Background. Th2 lymphocyte responses are associated with inflammation and disease during allergic responses. Exposure to particular environmental factors during the expression of allergy could result in more pronounced Th2-1ike immune responses and more severe disease. One factor might be a respiratory virus infection. Objective. The aim of our study was to investigate the influence of respiratory syncytial virus (RSV) infection on the expression of ovalbumin (OVA)-induced allergy in BALB/c mice. Methods. We determined OVA-specific IgE in serum, cytokine profiles and histopathological lesions in lungs of OVA-allergic mice after RSV infection. Results. OVA sensitization and challenge induced OVA-specific IgE in serum, Th2 cytokine mRNA expression, and mononuclear and eosinophilic inflammation in the lungs. RSV inoculation during the challenge period enhanced OVA-induced IL-4 and IL-5 mRNA expression in lung tissue. RSV further enhanced the OVA-induced hypertrophy of mucous cells and eosinophilic infiltration in lung tissue. Surprisingly, RSV infection decreased Th2 cytokine secretion and eosinophilic influx in bronchoalveolar lavage of OVA-allergic mice. Because inactivated RSV did not influence these responses, replication of RSV appeared essential for the modification of OVA-induced Th2 cytokine expression. RSV did not change OVA-specific IgE levels in serum. Furthermore, the RSV-induced IL-12 mRNA expression in lung tissue of OVA-allergic mice was diminished, but IFN-γ mRNA expression was not affected. Conclusion. RSV infection enhanced particular OVA-induced Th2 cytokine mRNA responses and pulmonary lesions in allergic mice and thus aggravated allergic respiratory disease.

Allergy, Eosinophils, IFN-γ, IL-12, IL-4, IL-5, Ovalbumin, RSV, Th1/Th2 cytokines,
Clinical and Experimental Allergy
Department of Virology

Barends, M, Boelen, A, de Rond, L.G.H, Kwakkel, J, Bestebroer, T.M, Dormans, J.A.M.A, … Kimman, T.G. (2002). Influence of respiratory syncytial virus infection on cytokine and inflammatory responses in allergic mice. Clinical and Experimental Allergy, 32(3), 463–471. doi:10.1046/j.1365-2222.2002.01317.x