Helicobacter species DNA has been detected in liver tissue of patients affected by primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC). To investigate a potential causative relation between Helicobacter species and PBC/PSC, we compared the presence of Helicobacter species-specific DNA in liver tissue of patients with PBC/PSC (n = 18/n = 13) with those of a control group of patients with various liver diseases with known cause (n = 29). A PCR with Helicobacter genus-specific 16S rRNA primers was performed on DNA isolated from paraffin embedded liver tissue. Control patients had hepatitis-B (n = 9), alcoholic cirrhosis (n = 14), or non-cirrhotic metabolic liver disease (n = 6). There was no significant difference between the incidence of Helicobacter spp.-specific DNA in PBC/PSC (9/31; 29%) and the control group (10/29; 34%). Sequence analysis confirmed Helicobacter spp. DNA. Because Helicobacter spp. DNA can be found in approximately one-third of all samples tested, it is unlikely that PSC and PBC are caused by Helicobacter infection.

Alcoholic cirrhosis, Helicobacter species, Hepatitis B virus infection, Metabolic liver diseases, Primary biliary cirrhosis, Primary sclerosing cholangitis
dx.doi.org/10.1016/j.femsim.2004.11.002, hdl.handle.net/1765/68273
F E M S Immunology and Medical Microbiology
Department of Pathology

Boomkens, H.R, de Rave, S, Pot, R.G, Egberink, H.F, Penning, C, Rothuizen, J, … Kusters, J.G. (2005). The role of Helicobacter spp. in the pathogenesis of primary biliary cirrhosis and primary sclerosing cholangitis. In F E M S Immunology and Medical Microbiology (Vol. 44, pp. 221–225). doi:10.1016/j.femsim.2004.11.002