T-cell responses to human endogenous retrovirus (HERV) K(HML-2) Gag and Env were mapped in HIV-1-infected subjects using 15mer peptides. Small peptide pools and high concentrations were used to maximize sensitivity. In the 23 subjects studied, only three bona fide HERV-K(HML-2)-specific responses were detected. At these high peptide concentrations, we detected falsepositive responses, three of which were mapped to an HIV-1 Gag peptide contaminant. Thus, HERV-K(HML-2) Gag- and Envspecific T-cell responses are infrequently detected by 15mer peptide mapping. Copyright

doi.org/10.1128/CVI.05583-11, hdl.handle.net/1765/68782
Clinical and Vaccine Immunology (Print)
Department of Neurology

Jones, R.B, John, V.M, Hunter, D.V, Martin, E, Mujib, S, Mihajlovic, V, … Ostrowski, M.A. (2012). Human endogenous retrovirus K(HML-2) Gag- and Env-specific T-cell responses are infrequently detected in HIV-1-infected subjects using standard peptide matrix-based screening. Clinical and Vaccine Immunology (Print), 19(2), 288–292. doi:10.1128/CVI.05583-11