Background: Previous studies showed that combining the Rf-1 and Rf-3 or Rf-4 QTLs of FHH induced synergistic interactions markedly enhancing renal susceptibility. The present study aimed to determine the presence of such interaction between the Rf-1 and Rf-5 QTLs. Methods: Renal damage susceptibility was assessed in Rf-1B, Rf-1B+5, Rf-1B+4 congenics and ACI control rats in four situations: two-kidney control (2K), unilateral nephrectomy (UNX), L-NAME-induced hypertension (2K+L-NAME) and UNX+L-NAME. Albuminuria (UAV) and systolic blood pressure (SBP) were measured during 18 weeks of follow-up. In separate experiments, renal autoregulation was assessed in 2K rats. Results: In all four situations, Rf-1B+4 rats developed more severe UAV than ACI, Rf-1B and Rf-1B+5. There were no significant differences in UAV between Rf-1B and Rf-1B+5 rats. In the 2K and UNX situation no differences in SBP were noted between all four strains. With 2K+L-NAME and UNX+L-NAME treatment, SBP in double congenics was higher than that of ACI and Rf-1B rats. Renal autoregulation was similarly impaired in all three congenic strains. Conclusion: We conclude that the Rf-5 region, alone or in the presence of Rf-1B, does not affect the development of renal damage. We cannot substantiate that the Rf-5 region contains genes influencing renal damage susceptibility. Copyright

Chronic kidney disease, Double congenic rats, Rats, renal susceptibility, Renal damage in rats, Renal failure-1 (Rf-1) through Rf-5, Rf-1 and Rf-5 QTLs,
Nephron - Experimental Nephrology
Department of Pediatric Surgery

van Dijk, S.J, Specht, P, Lazar, J, Jacob, H.J, & Provoost, A.P. (2006). Absence of an interaction between the Rf-1 and Rf-5 QTLs influencing susceptibility to renal damage in rats. Nephron - Experimental Nephrology, 104(3). doi:10.1159/000094548