We report on two-photon autofluorescence and second harmonic spectral imaging of live mouse tissues. The use of a high sensitivity detector and ultraviolet optics allowed us to record razor-sharp deep-tissue spectral images of weak autofluorescence and short-wavelength second harmonic generation by mouse skin. Real-color image representation combined with depth-resolved spectral analysis enabled us to identify tissue structures. The results show that linking nonlinear deep-tissue imaging microscopy with autofluorescence spectroscopy has the potential to provide important information for the diagnosis of skin tissues.

doi.org/10.1364/OE.14.004395, hdl.handle.net/1765/70337
Optics Express
Department of Radiation Oncology

Palero, J.A, de Bruijn, H.S, van der Ploeg-van den Heuvel, A, Sterenborg, H.J.C.M, & Gerritsen, H.C. (2006). In vivo nonlinear spectral imaging in mouse skin. Optics Express, 14(10), 4395–4402. doi:10.1364/OE.14.004395