2012
The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits
Publication
Publication
BMC Biochemistry , Volume 13 - Issue 1
Background: Mutations to the TSC1 and TSC2 genes cause the disease tuberous sclerosis complex. The TSC1 and TSC2 gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth. Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation. Results: TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration. Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits. Conclusions: TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.
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| , , , | |
| doi.org/10.1186/1471-2091-13-18, hdl.handle.net/1765/71287 | |
| BMC Biochemistry | |
| Organisation | Department of Clinical Genetics |
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Hoogeveen-Westerveld, M., van Unen, L., van den Ouweland, A., Halley, D., Hoogeveen, A., & Nellist, M. (2012). The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits. BMC Biochemistry, 13(1). doi:10.1186/1471-2091-13-18 |
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