Background: Mutations to the TSC1 and TSC2 genes cause the disease tuberous sclerosis complex. The TSC1 and TSC2 gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth. Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation. Results: TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration. Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits. Conclusions: TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.

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doi.org/10.1186/1471-2091-13-18, hdl.handle.net/1765/71287
BMC Biochemistry
Department of Clinical Genetics

Hoogeveen-Westerveld, M, van Unen, L, van den Ouweland, A.M.W, Halley, D.J.J, Hoogeveen, A.T, & Nellist, M.D. (2012). The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits. BMC Biochemistry, 13(1). doi:10.1186/1471-2091-13-18