For poultry as well as for mammalian species used for scientific research, many immunocytochemical techniques have been developed to investigate in detail the interaction between infectious micro-organisms and the nonspecific and specific immune systems of the host. In this review three techniques have been described with all technical details necessary to perform them correctly: (1) single immunocytochemical staining to detect the infectious micro-organisms in situ at their site of infection, (2) double immunocytochemical staining to visualize simultaneously the infectious micro- organism and the host cellular response to investigate their interactions, and (3) detection of plasma cells producing antibodies specific to the micro- organism. Of the three techniques the results are described when applied on chicken tissues infected with various micro-organisms, such as Marek's disease virus, chicken anemia virus, infectious bursal disease virus and Eimeria tenella. (C) 2000 Elsevier Science Ltd.

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Developmental and Comparative Immunology
Department of Immunology

Jeurissen, P., Claassen, E., Boonstra-Blom, A., Vervelde, L., & Janse, M. (2000). Immunocytochemical techniques to investigate the pathogenesis of infectious micro-organisms and the concurrent immune response of the host. Developmental and Comparative Immunology (Vol. 24, pp. 141–151). doi:10.1016/S0145-305X(99)00069-5