We report on a novel and simple light source for short-wavelength two-photon excitation fluorescence microscopy based on the visible nonsolitonic radiation from a photonic crystal fiber. We demonstrate tunability of the light source by varying the wavelength and intensity of the Ti:Sapphire excitation light source. The visible nonsolitonic radiation is used as an excitation light source for two-photon fluorescence microscopy of tryptophan powder.

doi.org/10.1364/OPEX.13.005363, hdl.handle.net/1765/71626
Optics Express
Department of Radiation Oncology

Palero, J., Boer, V., Vijverberg, J., Gerritsen, H., & Sterenborg, D. (2005). Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source. Optics Express, 13(14), 5363–5368. doi:10.1364/OPEX.13.005363