2001-04-05
In vivo binding properties of [Carbonyl-11C]WAY-100635: Effect of endogenous serotonin
Publication
Publication
Synapse , Volume 40 - Issue 2 p. 122- 129
[Carbonyl-11C]WAY-100635 has been reported to be a useful ligand for the investigation of 5-HT1A receptor imaging in vivo. However, the cellular distribution and the influence of endogenous serotonin (5-HT) on in vivo binding have not been fully examined. In this study, we investigated the effect of 5,7-dihydroxytryptamine-produced destruction of 5-HT neurons, reserpine-induced 5-HT depletion, and fenfluramine-induced 5-HT increase on [carbonyl-11C]WAY-100635 binding in vivo. There was no significant change in the uptake of [carbonyl-11C]WAY-100635 in the slice of 5-HT denervated rat brain except in the raphe nucleus, where 5-HT cell bodies exist. There was no obvious effect of enhanced 5-HT release by fenfluramine or decreased release by reserpine on [carbonyl-11C]WAY-100635 binding in the dissected brain region. No significant effect was observed in the time course of [carbonyl-11C]WAY-100635 in the hippocampus and frontal cortex measured by PET. These results indicated that the in vivo binding of [carbonyl-11C]WAY-100635 in the hippocampus and cerebral cortex mainly reflects postsynaptic 5-HT1A receptor binding, and that this binding is not sensitive to endogenous 5-HT.
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doi.org/10.1002/syn.1033, hdl.handle.net/1765/71816 | |
Synapse | |
Organisation | Department of Clinical Genetics |
de Vries, B., Tyson, M., Winter, R. M., & Malcolm, S. (2001). In vivo binding properties of [Carbonyl-11C]WAY-100635: Effect of endogenous serotonin. Synapse, 40(2), 122–129. doi:10.1002/syn.1033 |