We utilized a setup based on extensive pre-fractionation of proteolytic peptides and nanoflow reversed-phase LC-MS/MS to identify the (sub)proteome of human follicular fluid (FF). In this in-depth screen, 246 specific proteins were identified, the majority of which are involved in coagulation- and immune-response pathways. Our aim is to define a set of FF protein markers, which could predict oocyte quality.

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doi.org/10.1002/pmic.201100240, hdl.handle.net/1765/72247
Department of Gynaecology & Obstetrics

Twigt, J., Steegers-Theunissen, R., Bezstarosti, K., & Demmers, J. (2012). Proteomic analysis of the microenvironment of developing oocytes. Proteomics, 12(9), 1463–1471. doi:10.1002/pmic.201100240