Background: Treatment with a specific HSP60 epitope in new onset of type 1 diabetes (T1D) patients has been shown to preserve endogenous insulin production. Previously, recognition of pan HLA-DR-binding HSP60 epitopes in various autoimmune diseases was found; this study investigated recognition of these epitopes in newly diagnosed T1D patients and correlated findings to the occurrence of a partial remission. Methods: Peripheral blood mononuclear cells of 18 children with T1D were prospectively collected at disease onset and a few months after diagnosis. Epitope-specific T-cell proliferation and cytokine production (intracellular and in culture supernatants) were measured. Results were compared with 31 longstanding T1D patients and ten healthy controls. Results: Although HSP60 epitope-specific T-cell proliferative responses were detected, overall proliferative responses were low. At onset, epitope-specific intracellular IFN-γ production was higher in T1D patients compared with healthy controls (p<0.05). At follow-up, both IL-10 and IFN-γ production were higher in those without a partial remission than in those with a partial remission (both p<0.05). Also, IL-10 and IFN-γ production were higher compared with onset for patients without a PR (both p<0.01). In supernatants of HSP60 epitope-specific T-cell cultures, no substantial differences in cytokine production were found between T1D patients with and without a partial remission, either at onset or a few months after onset. As patient numbers were small, results should be interpreted with caution. Conclusions: Pan-DR-binding HSP60 peptides induced low peptide-specific proliferative responses and peptide-specific production of some, mainly intracellular, cytokines in T1D patients. Recognition did not differ significantly between patient groups and various time points.

Additional Metadata
Keywords Children, Cytokines, Heat shock protein 60 (HSP60), Type 1 diabetes
Persistent URL dx.doi.org/10.1002/dmrr.2306, hdl.handle.net/1765/72503
Journal Diabetes - Metabolism: Research and Reviews (Print)
Citation
Verrijn Stuart, A.A, de Jager, W, Klein, M, Teklenburg, G, Nuboer, R, Hoorweg-Nijman, J.J, … Prakken, B.J. (2012). Recognition of heat shock protein 60 epitopes in children with type 1 diabetes. Diabetes - Metabolism: Research and Reviews (Print), 28(6), 527–534. doi:10.1002/dmrr.2306