1997-07-04
Determination of the lactone and lactone plus carboxylate forms of 9-aminocamptothecin in human plasma by sensitive high-performance liquid chromatography with fluorescence detection
Publication
Publication
Journal of Chromatography B: Biomedical Applications , Volume 694 - Issue 2 p. 435- 441
Two sensitive reversed-phase high-performance liquid chromatographic fluorescence methods, with simple sample handling at the site of the patient, are described for the determination of the lactone and lactone plus carboxylate forms of g-aminocamptothecin (9AC). For 9AC lactone, the sample preparation was a liquid-liquid extraction with acetonitrile-n-butyl chloride (1:4, v/v), whereas the sample preparation for 9AC total (lactone plus carboxylate) was a simple deproteinization with 5% perchloric acid-methanol (1:1, v/v), which results in the conversion of the carboxylate into the lactone form. The lower limits of quantitation were 50 pg/ml and 100 pg/ml for 9AC lactone and 9AC total, respectively. The within-run precisions at four tested concentrations were ≤6.3% for 9AC lactone and ≤5.3% for 9AC total. The between-run precisions were ≤8.9% and ≤5.6%, respectively. The assays were developed to enable pharmacological analysis of 9AC in a bioavailability and oral phase I study in patients with solid tumors.
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doi.org/10.1016/S0378-4347(97)00117-5, hdl.handle.net/1765/73132 | |
Journal of Chromatography B: Biomedical Applications | |
Organisation | Department of Medical Oncology |
Loos, W., Sparreboom, A., Verweij, J., Nooter, K., Stoter, G., & Schellens, J. (1997). Determination of the lactone and lactone plus carboxylate forms of 9-aminocamptothecin in human plasma by sensitive high-performance liquid chromatography with fluorescence detection. Journal of Chromatography B: Biomedical Applications, 694(2), 435–441. doi:10.1016/S0378-4347(97)00117-5 |