2014-01-14
Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC
Publication
Publication
BMC Infectious Diseases , Volume 14 - Issue 1
Background: Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up of patients in an outbreak setting, a molecular screening method was developed for detection of the most prevalent carbapenemase genes; blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC.Methods: A real-time multiplex PCR assay was evaluated using a collection of 86 Gram negative isolates, including 62 carbapenemase producers. Seven different laboratories carried out this method and used the assay for detection of the carbapenemase genes on a selection of 20 isolates.Results: Both sensitivity and specificity of the multiplex PCR assay was 100%, as established by results on the strain collection and the inter-laboratory comparisons.Conclusions: In this study, we present a multiplex real-time PCR that is a robust, reliable and rapid method for the detection of the most prevalent carbapenemases blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC, and is suitable for screening of broth cultured rectal swabs and for identification of carbapenemase genes in cultures.
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| doi.org/10.1186/1471-2334-14-27, hdl.handle.net/1765/75166 | |
| BMC Infectious Diseases | |
| Organisation | Department of Medical Microbiology and Infectious Diseases |
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van der Zee, A., Roorda, L., Bosman, G., Fluit, A., Hermans, M., Smits, P., van der Zanden, A., te Witt, R., Bruijnesteijn van Coppenraet, L., Cohen Stuart, J.& Ossewaarde, J. (2014). Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC. BMC Infectious Diseases, 14(1).https://doi.org/10.1186/1471-2334-14-27 |
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