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M.P.C. van de Corput (Mariëtte), T.A. Knoch (Tobias), E. de Boer (Ernie), W.A. van Cappellen (Gert), M. Lesnussa (Michael) and H.J.F.M.M. Eussen (Bert)

2010-06-18

The 3D chromatin structure of the mouse β-haemoglobin gene cluster

Publication

Publication

Presented at the 10th international ELMI Meeting, EMBO Workshop on Advanced Light Microscopy Techniques and their Applications, EMBL Heidelberg (June 2010), Heidelberg, Germany

Here we show a 3D DNA-FISH method to visualizes the 3D structure of the β-globin locus. Geometric size and shape measurements of the 3D rendered signals (128Kb) show that the volume of the β-globin locus decreases almost two fold upon gene activation. A decrease in length and a distinctive change in shape and surface structure of the locus are also observed. Adding 5’ and 3’end regions to the probe (175Kb) showed a less prominent change in length, shape and structure. It was shown (data not on this poster) that the physical distance between the two flanking regions shift in a similar limited manner, indicating that the flanking regions do not participate in ACH formation and thus active chromatin folding is occurring only within the locus proper.

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Keywords Genome, genomics, genome organization, genome architecture, structural sequencing, architectural sequencing, systems genomics, coevolution, holistic genetics, genome mechanics, genome function, genetics, gene regulation, replication, transcription, repair, homologous recombination, simultaneous co-transfection, cell division, mitosis, metaphase, interphase, cell nucleus, nuclear structure, nuclear organization, chromatin density distribution, nuclear morphology, chromosome territories, subchromosomal domains, chromatin loop aggregates, chromatin rosettes, chromatin loops, chromatin fibre, chromatin density, persistence length, spatial distance measurement, histones, H1.0, H2A, H2B, H3, H4, mH2A1.2, DNA sequence, complete sequenced genomes, molecular transport, obstructed diffusion, anomalous diffusion, percolation, long-range correlations, fractal analysis, scaling analysis, exact yard-stick dimension, box-counting dimension, lacunarity dimension, local nuclear dimension, nuclear diffuseness, parallel super computing, grid computing, volunteer computing, Brownian Dynamics, Monte Carlo, fluorescence in situ hybridization, confocal laser scanning microscopy, fluorescence correlation spectroscopy, super resolution microscopy, spatial precision distance microscopy, auto- fluorescent proteins, CFP, GFP, YFP, DsRed, fusion protein, in vivo labelling, information browser, visual data base access, holistic viewing system, integrative data management, extreme visualization, three-dimensional virtual environment, virtual paper tool
Persistent URL hdl.handle.net/1765/77701
Conference 10th international ELMI Meeting, EMBO Workshop on Advanced Light Microscopy Techniques and their Applications, EMBL Heidelberg
Note Poster presented at the 10th international ELMI Meeting, EMBO Workshop on Advanced Light Microscopy Techniques and their Applications, EMBL Heidelberg, Heidelberg, Germany, 18th - 21th June, 2010
Organisation Biophysical Genomics, Department Cell Biology & Genetics
Citation
van de Corput, M., Knoch, T., de Boer, E., van Cappellen, G., Lesnussa, M., & Eussen, B. (2010). The 3D chromatin structure of the mouse β-haemoglobin gene cluster. Presented at the 10th international ELMI Meeting, EMBO Workshop on Advanced Light Microscopy Techniques and their Applications, EMBL Heidelberg. Retrieved from http://hdl.handle.net/1765/77701
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