Reproducibility of pharmacological ASL using sequences from different vendors: implications for multicenter drug studies
Magnetic Resonance Materials in Physics, Biology and Medicine , Volume 28 - Issue 5 p. 427- 436
Object: The current study assesses the multicenter feasibility of pharmacological arterial spin labeling (ASL) by comparing a caffeine-induced relative cerebral blood flow decrease (%CBF↓) measured with two pseudo-continuous ASL sequences as provided by two major vendors. Materials and methods: Twenty-two healthy volunteers were scanned twice with both a 3D spiral (GE) and a 2D EPI (Philips) sequence. The inter-session reproducibility was evaluated by comparisons of the mean and within-subject coefficient of variability (wsCV) of the %CBF↓, both for the total cerebral gray matter and on a voxel level. Results: The %CBF↓ was larger when measured with the 3D spiral sequence (23.9 ± 5.9 %) than when measured with the 2D EPI sequence (19.2 ± 5.6 %) on a total gray matter level (p = 0.02), and on a voxel level in the posterior watershed area (p < 0.001). There was no difference between the gray matter wsCV of the 3D spiral (57.3 %) and 2D EPI sequence (66.7 %, p = 0.3), whereas on a voxel level, the wsCV was visibly different between the sequences. Conclusion: The observed differences between ASL sequences of both vendors can be explained by differences in the employed readout modules. These differences may seriously hamper multicenter pharmacological ASL, which strongly encourages standardization of ASL implementations.
|Magnetic resonance imaging, Multicenter studies as topic, Perfusion, Pharmacological biomarkers, Reproducibility of results|
|Magnetic Resonance Materials in Physics, Biology and Medicine|
|Organisation||Department of Radiology|
Mutsaerts, H.-J, Steketee, R.M.E, Heijtel, D.F.R, Kuijer, J.P.A, van Osch, M.J.P, Majoie, C.B, … Nederveen, A.J. (2015). Reproducibility of pharmacological ASL using sequences from different vendors: implications for multicenter drug studies. Magnetic Resonance Materials in Physics, Biology and Medicine, 28(5), 427–436. doi:10.1007/s10334-014-0480-1