Aims: We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues. Materials & methods: SPIONs of different sizes and surface charges were exposed to fetal bovine serum. The structure/composition and biological effects of the protein corona-SPION complexes were probed. Results & discussion: The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs. In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells. Conclusion: Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.

LC-MS/MS, protein corona, SPIONs, surface charge,
Department of Gastroenterology & Hepatology

Mahmoudi, T, Sheibani, S, Milani, A.S, Rezaee, F, Gauberti, M, Dinarvand, R, & Vali, H. (2015). Crucial role of the protein corona for the specific targeting of nanoparticles. Nanomedicine, 10(2), 215–226. doi:10.2217/nnm.14.69