To gain more insight into initiation and regulation of T cell receptor (TCR) gene rearrangement during human T cell development, we analyzed TCR gene rearrangements by quantitative PCR analysis in nine consecutive T cell developmental stages, including CD34+ lin- cord blood cells as a reference. The same stages were used for gene expression profiling using DNA microarrays. We show that TCR loci rearrange in a highly ordered way (TCRD-TCRG-TCRB-TCRA) and that the initiating Ddelta2-Ddelta3 rearrangement occurs at the most immature CD34+CD38-CD1a- stage. TCRB rearrangement starts at the CD34+CD38+CD1a- stage and complete in-frame TCRB rearrangements were first detected in the immature single positive stage. TCRB rearrangement data together with the PTCRA (pTalpha) expression pattern show that human TCRbeta-selection occurs at the CD34+CD38+CD1a+ stage. By combining the TCR rearrangement data with gene expression data, we identified candidate factors for the initiation/regulation of TCR recombination. Our data demonstrate that a number of key events occur earlier than assumed previously; therefore, human T cell development is much more similar to murine T cell development than reported before.

Additional Metadata
Keywords Animals, Antigens, CD/genetics/immunology, Cell Differentiation/genetics/*immunology, Cells, Cultured, Gene Expression Profiling, Gene Expression Regulation/genetics/*immunology, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor/genetics/*immunology, Gene Rearrangement, delta-Chain T-Cell Antigen Receptor/genetics/*immunology, Genes, T-Cell Receptor beta/genetics/immunology, Humans, Mice, Oligonucleotide Array Sequence Analysis, Receptors, Antigen, T-Cell, gamma-delta/genetics/immunology, Research Support, Non-U.S. Gov't, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes/*immunology
Persistent URL hdl.handle.net/1765/8406
Journal The Journal of Experimental Medicine
Citation
Dik, W.A, van Dongen, J.J.M, Staal, F.J.T, Langerak, A.W, Weerkamp, F, de Ridder, D, … Pike, K. (2005). New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling. The Journal of Experimental Medicine. Retrieved from http://hdl.handle.net/1765/8406