PURPOSE. To evaluate the validity of a novel fully automated three-dimensional (3D) method capable of segmenting the choroid from two different optical coherence tomography scanners: swept-source OCT (SS-OCT) and spectral-domain OCT (SD-OCT). METHODS. One hundred eight subjects were imaged using SS-OCT and SD-OCT. A 3D method was used to segment the choroid and quantify the choroidal thickness along each A-scan. The segmented choroidal posterior boundary was evaluated by comparing to manual segmentation. Differences were assessed to test the agreement between segmentation results of the same subject. Choroidal thickness was defined as the Euclidian distance between Bruch’s membrane and the choroidal posterior boundary, and reproducibility was analyzed using automatically and manually determined choroidal thicknesses. RESULTS. For SS-OCT, the average choroidal thickness of the entire 6-by 6-mm<sup>2</sup> macular region was 219.5 lm (95% confidence interval [CI], 204.9–234.2 µm), and for SD-OCT it was 209.5 µm (95% CI, 197.9–221.0 µm). The agreement between automated and manual segmentations was high: Average relative difference was less than 5 lm, and average absolute difference was less than 15 µm. Reproducibility of choroidal thickness between repeated SS-OCT scans was high (coefficient of variation [CV] of 3.3%, intraclass correlation coefficient [ICC] of 0.98), and differences between SS-OCT and SD-OCT results were small (CV of 11.0%, ICC of 0.73). CONCLUSIONS. We have developed a fully automated 3D method for segmenting the choroid and quantifying choroidal thickness along each A-scan. The method yielded high validity. Our method can be used reliably to study local choroidal changes and may improve the diagnosis and management of patients with ocular diseases in which the choroid is affected.

Automated segmentation, Choroid, Quantification, Spectraldomain OCT, Swept-source OCT
dx.doi.org/10.1167/iovs.14-15669, hdl.handle.net/1765/85635
Investigative Ophthalmology & Visual Science
Department of Ophthalmology

Zhang, L, Buitendijk, G.H.S, Lee, K, Sonka, M, Springelkamp, H, Hofman, A, … Abràmoff, M.D. (2015). Validity of automated choroidal segmentation in SS-OCT and SD-OCT. Investigative Ophthalmology & Visual Science, 56(5), 3202–3211. doi:10.1167/iovs.14-15669