Genome maintenance by homologous recombination depends on coordinating many proteins in time and space to assemble at DNA break sites. To understand this process, we followed the mobility of BRCA2, a critical recombination mediator, in live cells at the single-molecule level using both single-particle tracking and fluorescence correlation spectroscopy. BRCA2-GFP and -YFP were compared to distinguish diffusion from fluorophore behavior. Diffusive behavior of fluorescent RAD51 and RAD54 was determined for comparison. All fluorescent proteins were expressed from endogenous loci. We found that nuclear BRCA2 existed in oligomeric clusters, and exhibited heterogeneous mobility. DNA damage increased BRCA2 transient binding, presumably including binding to damaged sites. Despite its very different size, RAD51 displayed mobility similar to BRCA2, which indicates physical interaction between these proteins both before and after induction of DNA damage. We propose that BRCA2-mediated sequestration of nuclear RAD51 serves to prevent inappropriate DNA interactions and that all RAD51 is delivered to DNA damage sites in association with BRCA2.

doi.org/10.1083/jcb.201405014, hdl.handle.net/1765/85640
The Journal of Cell Biology
Department of Radiation Oncology

Reuter, M, Zelensky, A, Smal, I, Meijering, H.W, van Cappellen, W.A, de Gruiter, H.M, … Wyman, C. (2014). BRCA2 diffuses as oligomeric clusters with RAD51 and changes mobility after DNA damage in live cells. The Journal of Cell Biology, 207(5), 599–613. doi:10.1083/jcb.201405014