To determine the postantibiotic effect of aminoglycosides, two methods are currently being used to remove the test drug: repeated washing and dilution. An enzymatic inactivation method of removing gentamicin and tobramycin was developed and compared with the dilution method. This enzymatic method provides a rapid and simple alternative method of removing aminoglycosides which results in reliable postantibiotic-effect values.

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Antimicrobial Agents and Chemotherapy
Erasmus MC: University Medical Center Rotterdam

den Hollander, J., Mouton, J., Bakker-Woudenberg, I., Vleggaar, F., van Goor, M.-L., & Verbrugh, H. (1996). Enzymatic method for inactivation of aminoglycosides during measurement of postantibiotic effect. Antimicrobial Agents and Chemotherapy. Retrieved from