Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas
We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.
|*DNA Primers, *Polymerase Chain Reaction, Base Sequence, DNA, Viral/*analysis, Female, Humans, Molecular Sequence Data, Papillomavirus, Human/*genetics, Research Support, Non-U.S. Gov't, Uterine Cervical Neoplasms/*virology|
|Journal of Clinical Microbiology|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Baay, M.F.D, Quint, W.G.V, Koudstaal, J, Hollema, H, Duk, J.M, Burger, M.P.M, … Herbrink, P. (1996). Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas. Journal of Clinical Microbiology. Retrieved from http://hdl.handle.net/1765/8635