We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

Department of Virology

Reusken, C., Mou, H., Godeke, G.-J., van der Hoek, L., Meyer, B., Müller, M., … Koopmans, M., D.V.M. (2013). Specific serology for emerging human coronaviruses by protein microarray. Eurosurveillance, 18(14), 2–7. Retrieved from http://hdl.handle.net/1765/87235