We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

This work was funded by the European Commission 7th Framework Programme; grant id fp7/223498 - European management platform for emerging and re-emerging infectious disease entities (EMPERIE)
Department of Virology

Reusken, C.B.E.M, Mou, H, Godeke, G-J, van der Hoek, L, Meyer, B, Müller, M.A, … Koopmans, M.P.G, D.V.M. (2013). Specific serology for emerging human coronaviruses by protein microarray. Eurosurveillance, 18(14), 2–7. Retrieved from http://hdl.handle.net/1765/87235