Chapter 1 is a brief introduction to the renal mechanisms and clinical disorders of water and sodium balance. The aims of the thesis are presented. The thesis is divided into two parts. Part A (Chapters 2 – 6) presents studies investigating the renal mechanisms of water and sodium balance regulation, while Part B (Chapters 7 – 14) presents studies investigating clinical disorders of water and sodium balance. Chapter 2, the first chapter of Part A, is an introduction to the proteomics technique Difference Gel Electrophoresis (DIGE). Proteomics is a promising new technique with the ability to study cellular signaling pathways and identify clinical disease biomarkers. DIGE is currently one of the few techniques to perform quantitative proteomics, generating a statistical output to differences in protein abundances. The chapter discusses the principles of DIGE-based proteomics, including sample preparation, two-dimensional electrophoresis (2-DE), statistical analysis of 2D-gels, and mass spectrometry. Strengths and weaknesses of DIGE are discussed, including possible solutions to overcome certain limitations, such as the identification of low abundance and transmembrane proteins. By giving a synopsis of our recent proteomics studies, we illustrate how DIGE may be used to study the renal mechanisms of water and sodium balance regulation. Finally, we illustrate how quantification based on the DIGE approach combined with bioinformatics may facilitate the study of systems biology of the kidney.

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Dutch Diabetes Research Foundation, Dutch Kidney Foundation (Nederlandse Nierstichting), Weimar, Prof. Dr. W. (promotor)
W. Weimar (Willem)
Erasmus University Rotterdam
hdl.handle.net/1765/8840
Erasmus MC: University Medical Center Rotterdam

Hoorn, E. (2007, February 28). Water and Salt: from renal mechanisms to clinical disorders. Retrieved from http://hdl.handle.net/1765/8840