An increased content of hydrogen peroxide (H2O2), a marker of inflammation, has been described in the condensate of exhaled air from adults and children with inflammatory lung disorders, including asthma. However, the normal range of [H2O2] in the exhaled air condensate from healthy children has not been established. Therefore, the aim of this study was to determine the reference range of exhaled [H2O2] in healthy school-aged children. Ninety-three healthy nonsmoking children (48 female and 45 male, mean age 10 yrs, range 8-13 yrs), with a negative history for allergy, eczema or respiratory disease and with a normal lung function, participated. Exhaled air condensate was examined fluorimetrically for the presence of H2O2. In addition, the reproducibility of [H2O2] within subjects and between days and the stability of [H2O2] during storage at -20 degrees C were assessed. The median [H2O2] in the exhaled air condensate of all children was 0.13 microM, with a 2.5-97.5% reference range of <0.01-0.48 microM. No significant difference existed between males and females. There was no correlation between exhaled [H2O2] and age or lung function. Repeated [H2O2] measurements on 2 consecutive days showed satisfactory within-subject reproducibility and [H2O2] in stored samples remained stable for at least 1 month at -20 degrees C. In conclusion, this study provides reference data for exhaled hydrogen peroxide in a large group of healthy children. The observed levels were lower than those reported previously for healthy adults and were independent of age, sex and lung function.

*Breath Tests, Child, Female, Humans, Hydrogen Peroxide/*analysis, Male, Reference Values, Reproducibility of Results, Research Support, Non-U.S. Gov't, Respiratory Function Tests
hdl.handle.net/1765/8896
The European Respiratory Journal
Erasmus MC: University Medical Center Rotterdam

Jobsis, Q, Raatgeep, R.H, Schellekens, S.L, Hop, W.C.J, Hermans, P.W.M, & de Jongste, J.C. (1998). Hydrogen peroxide in exhaled air of healthy children: reference values. The European Respiratory Journal. Retrieved from http://hdl.handle.net/1765/8896