TCRs genetically linked to CD28 and CD3ε Do not mispair with endogenous tcr chains and mediate enhanced T cell persistence and anti-melanoma activity
Journal of Immunology , Volume 193 - Issue 10 p. 5315- 5326
Adoptive transfer of T cells that are gene engineered to express a defined TCR represents a feasible and promising therapy for patients with tumors. However, TCR gene therapy is hindered by the transient presence and effectiveness of transferred T cells, which are anticipated to be improved by adequate T cell costimulation. In this article, we report the identification and characterization of a novel two-chain TCR linked to CD28 and CD3 (i.e., TCR:28ε). This modified TCR demonstrates enhanced binding of peptide-MHC and mediates enhanced T cell function following stimulation with peptide compared with wild-Type TCR. Surface expression of TCR:28ε depends on the transmembrane domain of CD28, whereas T cell functions depend on the intracellular domains of both CD28 and CD3ε, with IL-2 production showing dependency on CD28:LCK binding. TCR:28ε, but not wild-Type TCR, induces detectable immune synapses in primary human T cells, and such immune synapses show significantly enhanced accumulation of TCR transgenes and markers of early TCR signaling, such as phosphorylated LCK and ERK. Importantly, TCR:28ε does not show signs of off-Target recognition, as evidenced by lack of TCR mispairing, as well as preserved specificity. Notably, when testing TCR:28ε in immune-competent mice, we observed a drastic increase in T cell survival, which was accompanied by regression of large melanomas with limited recurrence. Our data argue that TCR transgenes that contain CD28, and, thereby, may provide T cell costimulation in an immune-suppressive environment, represent candidate receptors to treat patients with tumors.
|Journal of Immunology|
|Organisation||Department of Medical Oncology|
Govers, C.C.F.M, Sebestyén, Z, Roszik, J, van Brakel, M, Berrevoets, C.A, Szöor, Á, … Debets, J.E.M.A. (2014). TCRs genetically linked to CD28 and CD3ε Do not mispair with endogenous tcr chains and mediate enhanced T cell persistence and anti-melanoma activity. Journal of Immunology, 193(10), 5315–5326. doi:10.4049/jimmunol.1302074