Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples.

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Diagnostic Microbiology and Infectious Disease
Department of Medical Microbiology and Infectious Diseases

Stappers, M.H.T, Hagen, F, Reimnitz, P, Mouton, J.W, Meis, J.F, & Gyssens, I.C. (2016). Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: Direct molecular versus culture-based detection. Diagnostic Microbiology and Infectious Disease, 85(2), 263–265. doi:10.1016/j.diagmicrobio.2015.12.017