The dynamic three-dimensional chromatin architecture of genomes and its coevolutionary connection to its function – the storage, expression, and replication of genetic information – is still one of the central issues in biology. Here, we describe the much debated 3D-architecture of the human and mouse genomes from the nucleosomal to the megabase pair level by a novel approach combining selective high-throughput high-resolution chromosomal interaction capture (T2C), polymer simulations, and scaling analysis of the 3D-architecture and the DNA sequence: The genome is compacted into a chromatin quasi-fibre with ~5±1 nucleosomes/11nm, folded into stable ~30-100 kbp loops forming stable loop aggregates/rosettes connected by similar sized linkers. Minor but significant variations in the architecture are seen between cell types/functional states. The architecture and the DNA sequence show very similar finestructured multi-scaling behaviour confirming their co-evolution and the above. This architecture, its dynamics, and accessibility balance stability and flexibility ensuring genome integrity and variation enabling gene expression/regulation by self-organization of (in)active units already in proximity. Our results agree with the heuristics of the field and allow "architectural sequencing" at a genome mechanics level to understand the inseparable systems genomic properties.

, , , , , , , ,
Biophysical Genomics, Department Cell Biology & Genetics

Knoch, T., Wachsmuth, M., Kepper, N., Lesnussa, M., Abuseiris, A., Imam, A., … Grosveld, F. (2016). The Detailed 3D Multi-Loop Aggregate/Rosette Chromatin Architecture and Functional Dynamic Organization of the Human and Mouse Genomes. - BioRxiv Version. Retrieved from

Additional Files
TAKnoch-Fig01-A-F-3DT2C-Method-IntMaps-3DModel-140921.eps.gz Final Version , 43mb
TAKnoch-Fig02-A-E-3DT2C-Scaling-140818.eps.gz Final Version , 3mb
TAKnoch-SFig01-A-F-3DT2C-Gels-HS-140628.eps.gz Final Version , 1mb
TAKnoch-SFig02-A-F-3DT2C-Gels-MM-140628.eps.gz Final Version , 1mb
TAKnoch-SFig03-A-B-3DT2C-FragmentDistributions-140628.eps.gz Final Version , 163kb
TAKnoch-SFig04-A-E-3DT2C-MatrixStatisticalLimit-140814.eps.gz Final Version , 10mb
TAKnoch-SFig05-A-F-3DT2C-MethodComparison-HS-140815.eps.gz Final Version , 503kb
TAKnoch-SFig06-A-F-3DT2C-MethodComparison-MM-140916.eps.gz Final Version , 493kb
TAKnoch-SFig07-3DT2C-SimilarityOfMatrixProjections-140814.eps.gz Final Version , 252kb
TAKnoch-SFig08-A-E-3DT2C-DetailedHSMMScaling-140815.eps.gz Final Version , 333kb
TAKnoch-SFig09-A-C-3DT2C-HighResScalingFineStruct-140630.eps.gz Final Version , 179kb
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TAKnoch-SFig11-A-H-3DT2C-SimulatedDistInteractionMapsMLS-140628.eps.gz Final Version , 306mb
TAKnoch-SFig12-A-H-3DT2C-SimulatedDistInteractionMapsRWGL-140628.eps.gz Final Version , 238mb
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TAKnoch-SFig16-A-F-3DT2C-C-CorrAvgHumanMouseExpCompErr-140628.eps.gz Final Version , 3mb
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TAKnoch-SFig21-A-B-3DT2C-CorrAvgFineStruct-140628.eps.gz Final Version , 386kb Final Version , 14mb
TAKnoch-SMov02-IGF-EndLinkers-20nm-146ms.mpg Final Version , 36mb
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