1987
Role of splenic stroma in the action of bacterial lipopolysaccharides on radiation mortality: a study in mice carrying the Slj allele
Publication
Publication
Cell Proliferation , Volume 20 - Issue 1 p. 29- 36
Abstract. Slj/+ mice display a slight macrocytic anaemia due to a defect in their haemopoietic organ stroma. They have a deficient endogenous spleen colony (CFU‐end) formation following sublethal doses of gamma‐radiation compared with their normal +/+ littermates, which is likely to be due to the low pre‐irradiation CFU‐S content of the Slj/+ spleen. CFU‐S in these congenic mice do not differ in their sensitivity to gamma‐irradiation or stem cell‐activating factor. While injection of +/+ mice with 10 μg of lipopolysaccharide‐W (LPS) one day prior to irradiation led to a substantial increase in their survival, the survival of Slj/+ mice was only slightly increased. Irradiation induced a similar dose‐related reduction in the numbers of CFU‐S in the spleen and femora of LPS‐injected Slj/+ mice compared to similarly treated +/+ mice when measured directly after irradiation. At Day 9 after irradiation, injection of LPS led to a significantly higher CFU‐end formation and higher numbers of CFU‐S and nucleated cells in the Slj/+ spleens compared to LPS‐injected +/+ mice. No such differences in the radioprotective effect of LPS were observed in the +/+ and Slj/+ mice with respect to the splenic and femoral 59Fe‐incorporation and the femoral CFU‐S numbers at Day 9. These data strongly suggest a contribution by immigrating CFU‐S to the CFU‐S numbers and endogenous colony formation in at least the Slj/+ spleen after LPS injection and subsequent sublethal irradiation. The observations also imply that the splenic organ stroma may play a mediatory role in the radioprotective action of LPS. In addition, the data represent an extreme example of a lack of correlation between animal survival and haemopoietic parameters. Caution should be taken when applying endogenous colony counts as a means of screening potential anti‐radiation drugs. Copyright
| Additional Metadata | |
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| doi.org/10.1111/j.1365-2184.1987.tb01079.x, hdl.handle.net/1765/93605 | |
| Cell Proliferation | |
| Organisation | Biophysical Genomics, Department Cell Biology & Genetics |
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Ploemacher, R., & Brons, N. (1987). Role of splenic stroma in the action of bacterial lipopolysaccharides on radiation mortality: a study in mice carrying the Slj allele. Cell Proliferation, 20(1), 29–36. doi:10.1111/j.1365-2184.1987.tb01079.x |
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