Cohesin is essential for the maintenance of chromosomes through the cell cycle. In addition, cohesin contributes to the regulation of gene expression and the organization of chromatin in interphase cells. To study cohesin’s role in gene expression and chromatin organization, it is necessary to avoid secondary effects due to disruption of vital cohesin functions in the cell cycle. Here we describe experimental approaches to achieve this and the methods applied to define cohesin’s role in interphase.

Additional Metadata
Keywords Chromatin immunoprecipitation (ChIP), Chromosome conformation capture (3C), Cohesin, Conditional knockout, Gene expression, Quantitative real-time PCR (qRTPCR), Quantitative Western blot
Persistent URL dx.doi.org/10.1007/978-1-4939-6545-8_12, hdl.handle.net/1765/94215
Series Methods in Molecular Biology
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Citation
Gupta, P, Lavagnolli, T, Mira-Bontenbal, H, & Merkenschlager, M. (2017). Analysis of cohesin function in gene regulation and chromatin organization in interphase. In Cohesin and Condensin : Methods and Protocols (pp. 197–216). doi:10.1007/978-1-4939-6545-8_12