In this study we present a combined optical sizing and acoustical characterization technique for the study of the dynamics of single freely-floating ultrasound contrast agent microbubbles exposed to long burst ultrasound excitations up to the milliseconds range. A co-axial flow device was used to position individual microbubbles on a streamline within the confocal region of three ultrasound transducers and a high-resolution microscope objective. Bright-field images of microbubbles passing through the confocal region were captured using a high-speed camera synchronized to the acoustical data acquisition to assess the microbubble response to a 1-MHz ultrasound burst. Nonlinear bubble vibrations were identified at a driving pressure as low as 50 kPa. The results demonstrate good agreement with numerical simulations based on the shell-buckling model proposed by Marmottant et al. [J. Acoust. Soc. Am. 118, 3499-3505 (2005)]. The system demonstrates the potential for a high-throughput in vitro characterization of individual microbubbles.,
Applied Physics Letters
Erasmus MC: University Medical Center Rotterdam

Luan, Y., Renaud, G., Raymond, J. L., Segers, T., Lajoinie, G., Beurskens, R., … de Jong, N. (2016). Combined optical sizing and acoustical characterization of single freely-floating microbubbles. Applied Physics Letters, 109(23). doi:10.1063/1.4971391