We compared EUCAST and CLSI methods versus Etest for antifungal susceptibility testing of 20 clinically relevant Fusarium species against amphotericin B, posaconazole, and voriconazole. The median Etest amphotericin B and posaconazole MICs were 1 dilution higher than the median EUCAST and the CLSI MICs. The essential agreement (within ±1/±2 dilutions) was 60/90%, 80/95%, and 70/85% between the Etest and EUCAST methods and 80/95%, 75/95%, and 45/100% between the Etest and CLSI methods for amphotericin B, voriconazole, and posaconazole, respectively. The categorical agreement was >85%. Etest can be used for antifungal susceptibility testing of Fusarium species.

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Keywords Amphotericin B, Antifungal susceptibility, CLSI, Comparison, Etest, EUCAST, Fusarium, Posaconazole, RPB2, TEF1, Voriconazole
Persistent URL dx.doi.org/10.1128/AAC.01671-16, hdl.handle.net/1765/95446
Journal Antimicrobial Agents and Chemotherapy
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Citation
Al-Hatmi, A.M.S, Normand, A.-C. (Anne-Cecile), Ranque, S. (Stephane), Piarroux, R. (Renaud), de Hoog, G.S, Meletiadis, J, & Meis, J.F. (2017). Comparative evaluation of etest, EUCAST, and CLSI methods for amphotericin B, voriconazole, and posaconazole against clinically relevant fusarium species. Antimicrobial Agents and Chemotherapy, 61(1). doi:10.1128/AAC.01671-16