ZC3H12A, which encodes the RNase monocyte chemotactic protein-induced protein 1 (MCPIP1), is up-regulated in psoriatic skin and reduced to normal levels after clinical treatments with anti–IL-17A/IL-17R neutralizing antibodies. In IL-17A–stimulated keratinocytes, MCPIP1 is rapidly increased at the transcript and protein levels. Also, IL-17A was found to be the main inducer of ZC3H12A expression in keratinocytes treated with supernatants derived from a Streptococcus pyogenes-activated psoriatic ex vivo model based on the co-culture of psoriatic cutaneous lymphocyte-associated antigen (CLA+) T cells and lesional epidermal cells. Moreover, MCPIP1 was aberrantly distributed in the suprabasal layers of psoriatic epidermis. In psoriatic samples, IL-17A–stimulated epidermal cell suspensions showed an increased MCPIP1 expression, especially in the mid-differentiated cellular compartment. The knockdown of ZC3H12A showed that this RNase participates in the regulation of the mRNAs present in suprabasal differentiated keratinocytes. Furthermore, JAK/STAT3 inhibition prevented the IL-17A–dependent induction of MCPIP1. In the mouse model of imiquimod-induced psoriasis, Zc3h12a expression was abrogated in Il17ra–/– mice. These results support the notion that IL-17A–mediated induction of MCPIP1 is involved in the regulation of local altered gene expression in suprabasal epidermal layers in psoriasis.

doi.org/10.1016/j.jid.2016.04.030, hdl.handle.net/1765/95979
The Journal of Investigative Dermatology
Department of Dermatology

Ruiz-Romeu, E. (Ester), Ferran, M. (Marta), Giménez-Arnau, A., Bugara, B. (Beata), Lipert, B. (Barbara), Jura, J. (Jolanta), … Santamaria-Babí, L. F. (2016). MCPIP1 RNase Is Aberrantly Distributed in Psoriatic Epidermis and Rapidly Induced by IL-17A. The Journal of Investigative Dermatology, 136(8), 1599–1607. doi:10.1016/j.jid.2016.04.030