2016-07-29
Mumps-specific cross-neutralization by MMR vaccine-induced antibodies predicts protection against mumps virus infection
Publication
Publication
Vaccine , Volume 34 - Issue 35 p. 4166- 4171
Background Similar to other recent mumps genotype G outbreaks worldwide, most mumps patients during the recent mumps genotype G outbreaks in the Netherlands had received 2 doses of measles, mumps and rubella (MMR) vaccine during childhood. Here, we investigate the capacity of vaccine-induced antibodies to neutralize wild type mumps virus strains, including mumps virus genotype G. Methods In this study, we tested 105 pre-outbreak serum samples from students who had received 2 MMR vaccine doses and who had no mumps virus infection (n = 76), symptomatic mumps virus infection (n = 10) or asymptomatic mumps virus infection (n = 19) during the mumps outbreaks. In all samples, mumps-specific IgG concentrations were measured by multiplex immunoassay and neutralization titers were measured against the Jeryl Lynn vaccine strain and against wild type genotype G and genotype D mumps virus strains. Results The correlation between mumps-specific IgG concentrations and neutralization titers against Jeryl Lynn was poor, which suggests that IgG concentrations do not adequately represent immunological protection against mumps virus infection by antibody neutralization. Pre-outbreak neutralization titers in infected persons were significantly lower against genotype G than against the vaccine strain. Furthermore, antibody neutralization of wild type mumps virus genotype G and genotype D was significantly reduced in pre-outbreak samples from infected persons as compared with non-infected persons. No statistically significant difference was found for the vaccine strain. The sensitivity/specificity ratio was largest for neutralization of the genotype G strain as compared with the genotype D strain and the vaccine strain. Conclusions The reduced neutralization of wild type mumps virus strains in MMR vaccinated persons prior to infection indicates that pre-outbreak mumps virus neutralization is partly strain-specific and that neutralization differs between infected and non-infected persons. Therefore, we recommend the use of wild type mumps virus neutralization assays as preferred tool for surveillance of protection against mumps virus infection.
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doi.org/10.1016/j.vaccine.2016.06.063, hdl.handle.net/1765/97672 | |
Vaccine | |
Organisation | Department of Virology |
Gouma, S., ten Hulscher, H. I., Schurink-van ’t Klooster, T.M. (Tessa M.), de Melker, H.E. (Hester E.), Boland, G., Kaaijk, P., … van Binnendijk, R. (2016). Mumps-specific cross-neutralization by MMR vaccine-induced antibodies predicts protection against mumps virus infection. Vaccine, 34(35), 4166–4171. doi:10.1016/j.vaccine.2016.06.063 |