AIM: To establish if coincidental HLA-A, HLA-B, and HLA-DR tissue matching is associated with a reduced likelihood of corneal graft rejection. METHODS: Organ culture preserved random donor corneas were used for penetrating keratoplasty (PKP). Corneal tissue from all graft recipients and donors or blood samples from recipients after repeated transplantation were obtained in order to perform retrospective molecular HLA typing. A group of 21 recipients with a rejection episode (cases) after corneal transplantation was compared with a control group of non-rejectors (n = 43). 31 graft recipients were considered as high risk patients. The influence of HLA-A, HLA-B, and HLA-DR matching on rejection free graft survival time was analysed with Kaplan-Meyer statistics and Cox regression. RESULTS: A prolonged rejection free survival time was observed in graft recipients with one or two HLA-A matches (log rank test, p = 0.034). This effect was also observed in high risk graft recipients with one or two HLA-DR matches (log rank test, p = 0.030). CONCLUSIONS: Coincidental HLA-A and HLA-DR matches were observed and associated with a prolonged rejection free survival time in the total group and in the high risk group, respectively. These results support the beneficial effect of prospective HLA-A and HLA-DR typing upon corneal graft survival.

*Histocompatibility Testing, *Keratoplasty, Penetrating, Adolescent, Adult, Aged, Aged, 80 and over, Case-Control Studies, Child, Confidence Intervals, Female, Graft Rejection/*immunology, Graft Survival, HLA-A Antigens/*immunology, HLA-B Antigens/*immunology, HLA-DR Antigens/*immunology, Humans, Male, Middle aged, Odds Ratio, Proportional Hazards Models, Retrospective Studies
hdl.handle.net/1765/9777
British Journal of Ophthalmology: a peer review journal for health professionals and researchers in ophthalmology
Erasmus MC: University Medical Center Rotterdam

Bartels, M.C, Otten, H.G, van Gelderen, B.E, & van der Lelij, A. (2001). Influence of HLA-A, HLA-B, and HLA-DR matching on rejection of random corneal grafts using corneal tissue for retrospective DNA HLA typing. British Journal of Ophthalmology: a peer review journal for health professionals and researchers in ophthalmology. Retrieved from http://hdl.handle.net/1765/9777