Structure of Campylobacter jejuni lipopolysaccharides determines antiganglioside specificity and clinical features of Guillain-Barré and Miller Fisher patients
Ganglioside mimicry in the lipopolysaccharide (LPS) fraction of Campylobacter jejuni isolated from Guillain-Barre syndrome (GBS) and Miller Fisher syndrome (MFS) patients was compared with isolates from patients with an uncomplicated enteritis. The antibody response to C. jejuni LPS and gangliosides in neuropathy patients and controls was compared as well. LPS from GBS and MFS-associated isolates more frequently contained ganglioside-like epitopes compared to control isolates. Almost all neuropathy patients showed a strong antibody response against LPS and multiple gangliosides in contrast to enteritis patients. Isolates from GBS patients more frequently had a GM1-like epitope than isolates from MFS patients. GQ1b-like epitopes were present in all MFS-associated isolates and was associated with anti-GQ1b antibody reactivity and the presence of oculomotor symptoms. These results demonstrate that the expression of ganglioside mimics is a risk factor for the development of post-Campylobacter neuropathy. This study provides additional evidence for the hypothesis that the LPS fraction determines the antiganglioside specificity and clinical features in post-Campylobacter neuropathy patients.
|Antibodies, Bacterial/blood, Bacterial Typing Techniques, Campylobacter Infections/complications, Campylobacter jejuni/*chemistry/classification/immunology, Carbohydrate Sequence, Gangliosides/*immunology, Guillain-Barre Syndrome/*etiology/microbiology, Humans, Lipopolysaccharides/*chemistry/immunology, Miller Fisher Syndrome/*etiology/microbiology, Molecular Mimicry, Molecular Sequence Data, Research Support, Non-U.S. Gov't, Serotyping|
|Infection and Immunity|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Ang, C.W, Laman, J.D, Willison, H.J, Wagner, E.R, Endtz, H.P, de Klerk, M.A, … van Doorn, P.A. (2002). Structure of Campylobacter jejuni lipopolysaccharides determines antiganglioside specificity and clinical features of Guillain-Barré and Miller Fisher patients. Infection and Immunity. Retrieved from http://hdl.handle.net/1765/9849