university website

Lim, A.I. (Ai Ing), Li, Y. (Yan), Lopez-Lastra, S. (Silvia), R. Stadhouders (Ralph), Paul, F. (Franziska), Casrouge, A. (Armanda), N. Serafini (Nicolas), Puel, A. (Anne), Bustamante, J. (Jacinta), Surace, L. (Laura), et al. Masse-Ranson, G. (Guillemette), David, E. (Eyal), Strick-Marchand, H. (Helene), Le Bourhis, L. (Lionel), Cocchi, R. (Roberto), Topazio, D. (Davide), Graziano, P. (Paolo), Muscarella, L.A. (Lucia Anna), Rogge, L. (Lars), Norel, X. (Xavier), Sallenave, J.-M. (Jean-Michel), M. Allez (Matthieu), Graf, T. (Thomas), R.W. Hendriks (Rudi), J.-L. Casanova (Jean-Laurent), Amit, I. (Ido), Yssel, H. (Hans) and J.P. di Santo (James)

2017-03-09

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation

Publication

Publication

Cell , Volume 168 - Issue 6 p. 1086- 1100.e10

Innate lymphoid cells (ILCs) represent innate versions of T helper and cytotoxic T cells that differentiate from committed ILC precursors (ILCPs). How ILCPs give rise to mature tissue-resident ILCs remains unclear. Here, we identify circulating and tissue ILCPs in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration. Human ILCPs robustly generate all ILC subsets in vitro and in vivo. While human ILCPs express low levels of retinoic acid receptor (RAR)-related orphan receptor C (RORC) transcripts, these cells are found in RORC-deficient patients and retain potential for EOMES+ natural killer (NK) cells, interferon gamma-positive (IFN-γ+) ILC1s, interleukin (IL)-13+ ILC2s, and for IL-22+, but not for IL-17A+ ILC3s. Our results support a model of tissue ILC differentiation (“ILC-poiesis”), whereby diverse ILC subsets are generated in situ from systemically distributed ILCPs in response to local environmental signals.

Additional Metadata
Keywords cell fate, cytokines, development, humanized mice, innate lymphoid cells, lymphopoiesis, signaling, single cell cloning, transcription factors
Persistent URL doi.org/10.1016/j.cell.2017.02.021, hdl.handle.net/1765/98542
Journal Cell
Grant This work was funded by the European Commission 7th Framework Programme; grant id fp7/317057 - Host-microbe interactions in health and disease. Interface with the immune system (HOMIN), This work was funded by the European Commission 7th Framework Programme; grant id fp7/305578 - Pathogen COinfection: HIV, Tuberculosis, Malaria and Hepatitis C virus (PATHCO)
Organisation Department of Pulmonology
Citation
Lim, A.I. (Ai Ing), Li, Y. (Yan), Lopez-Lastra, S. (Silvia), Stadhouders, R., Paul, F. (Franziska), Casrouge, A. (Armanda), … di Santo, J. (2017). Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation. Cell, 168(6), 1086–1100.e10. doi:10.1016/j.cell.2017.02.021


User Publication Person Organisation Collection
Close