Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20. M. pneumoniae genomes per reaction.

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Keywords Detection, Multi-center evaluation, Mycoplasma pneumoniae, Real-time PCR
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Journal Diagnostic Microbiology and Infectious Disease
Dumke, R, Benitez, A.J. (Alvaro J.), Chalker, V. (Victoria), Gullsby, K. (Karolina), Henrich, B. (Birgit), Hidalgo-Grass, C. (Carlos), … Bebear, C. (Cecile). (2016). Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae. Diagnostic Microbiology and Infectious Disease. doi:10.1016/j.diagmicrobio.2017.03.004