The main goal of this thesis is to provide a solid foundation for further development of Raman spectroscopic applications in head and neck oncology. This is a multistep process in which we want to investigate and understand the Raman spectroscopic features that distinguish the epithelial (keratinocytic) component of a tongue OCSCC from its surrounding tissue (referred to as non-tumorous or healthy tissue).

First, we aimed to develop a standardized method to collect and analyze detailed Raman spectral information of OCSCC and the individual non-tumorous tissue structures in the tongue, as described in Chapter 2. Next, the potential of Raman spectroscopy to distinguish between the characteristic Raman features of OCSCC and the individual non-tumorous tissue structures is investigated in Chapter 3. In Chapter 4, these results are used for the development of a ‘tumor’ versus ‘non-tumorous tissue’ classification model. Lastly, in Chapter 5 the Raman spectra of OCSCC and individual non-tumorous tissue structures are analyzed in terms of the quantitative information they contain about the tissue’s molecular composition. In Chapter 6 an overview is given of the pure-compound reference spectra used for this analysis.
To conclude, in this thesis the strengths and limitations of Raman spectroscopy, regarding its OCSCCdetecting potential in tongue, are elucidated.

Additional Metadata
Promotor R.J. Baatenburg de Jong (Robert Jan) , S. Koljenović (Senada) , G.J. Puppels (Gerwin)
Publisher Erasmus University Rotterdam
ISBN 978-94-028-0590-1
Persistent URL
Cals, F.L.J. (2017, May 3). Raman Spectroscopy to Distinguish Tongue Cancer From Healthy Tissue. Erasmus University Rotterdam. Retrieved from