In vitro antifungal susceptibility testing of Candida isolates with the EUCAST methodology, a new method for ECOFF determination
The in vitro susceptibilities of 1,099 molecularly identified clinical Candida isolates against 8 antifungal drugs were determined using the EUCAST microdilution method. A new simple, objective, and mathematically solid method for determining epidemiological cutoff values (ECOFFs) was developed by derivatizing the MIC distribution and determining the derivatized ECOFF (dECOFF) as the highest MIC with the maximum second derivative. The dECOFFs were similar (95% agreement within 1 dilution) to the EUCAST ECOFFs. Overall, low non-wild-type/resistance rates were found. The highest rates were found for azoles with C. parapsilosis (2.7 to 9.8%), C. albicans (7%), and C. glabrata (1.7 to 2.3%) and for echinocandins with C. krusei (3.3%), C. albicans (1%), and C. tropicalis (1.7%).
|Keywords||Antifungal susceptibility testing, Candida, Epidemiological cutoff value, EUCAST|
|Persistent URL||dx.doi.org/10.1128/AAC.02372-16, hdl.handle.net/1765/99085|
|Journal||Antimicrobial Agents and Chemotherapy|
|Note||no subscription; no full text available yet|
Meletiadis, J, Curfs-Breuker, I, Meis, J.F, & Mouton, J.W. (2017). In vitro antifungal susceptibility testing of Candida isolates with the EUCAST methodology, a new method for ECOFF determination. Antimicrobial Agents and Chemotherapy, 61(4). doi:10.1128/AAC.02372-16