Obesity is a well-known risk factor for osteoarthritis, but it is unknown what it does on cartilage repair. Here we investigated whether a high fat diet (HFD) influences cartilage repair in a mouse model of cartilage repair. We fed DBA/1 mice control or HFD (60% energy from fat). After 2 weeks, a full thickness cartilage defect was made in the trochlear groove. Mice were sacrificed, 1, 8, and 24 weeks after operation. Cartilage repair was evaluated on histology. Serum glucose, insulin and amyloid A were measured 24h before operation and at endpoints. Immunohistochemical staining was performed on synovium and adipose tissue to evaluate macrophage infiltration and phenotype. One week after operation, mice on HFD had defect filling with fibroblast-like cells and more cartilage repair as indicated by a lower Pineda score. After 8 weeks, mice on a HFD still had a lower Pineda score. After 24 weeks, no mice had complete cartilage repair and we did not detect a significant difference in cartilage repair between diets. Bodyweight was increased by HFD, whereas serum glucose, amyloid A and insulin were not influenced. Macrophage infiltration and phenotype in adipose tissue and synovium were not influenced by HFD. In contrast to common wisdom, HFD accelerated intrinsic cartilage repair in DBA/1 mice on the short term. Resistance to HFD induced inflammatory and metabolic changes could be associated with accelerated cartilage repair.

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Keywords Animal model, Cartilage repair, High fat diet, Inflammation, Obesity
Persistent URL dx.doi.org/10.1002/jor.23280, hdl.handle.net/1765/99676
Journal Journal of Orthopaedic Research: a journal for musculoskeletal investigation
Wei, W, Bastiaansen-Jenniskens, Y.M, Suijkerbuijk, M. (Mathijs), Kops, N, Bos, P.K, Verhaar, J.A.N, … van Osch, G.J.V.M. (2017). High fat diet accelerates cartilage repair in DBA/1 Mice. Journal of Orthopaedic Research: a journal for musculoskeletal investigation, 35(6), 1258–1264. doi:10.1002/jor.23280