Acute rejection is one of the major immunological determinants of kidney graft function and survival. Early biomarkers to predict rejection are lacking. Emerging evidence reveals a crucial role for the monocyte/macrophage lineage cells in the pathogenesis of rejection. We hypothesized that higher pretransplant numbers of proinflammatory CD16+ monocytes can predict rejection. The study cohort consisted of 104 kidney transplant recipients (58 with no rejection and 46 with biopsy-proven rejection) and 33 healthy persons. Posttransplant median follow-up time was 14.7 mo (interquartile range 0.3-34 mo). Pretransplantation blood samples were analyzed by flow cytometry for monocyte immunophenotypes. Groups were compared by Cox regression models for the occurrence of acute rejection. We documented a significantly increased absolute number of pretransplant CD16+ monocytes in patients who developed biopsy-proven rejection after transplantation compared with those with no rejection (hazard ratio [HR] 1.60, 95% CI 1.28-2.00, p < 0.001) and healthy persons (HR 1.47, 95% CI 1.18-1.82, p < 0.001). In parallel, significantly fewer absolute numbers of CD16- monocytes were observed at pretransplant time points in rejectors versus nonrejectors (HR 0.74, 95% CI 0.58-0.94, p < 0,014). A higher pretransplant number of CD16+ monocytes is significantly associated with a higher risk of acute rejection after kidney transplantation.

Additional Metadata
Keywords Basic (laboratory) research/science, Flow cytometry, Immunobiology, Kidney (allograft) function/dysfunction, Kidney transplantation/nephrology, Macrophage/monocyte biology: activation, Macrophage/monocyte biology: trafficking, Pathology/histopathology, Rejection, Translational research/science
Persistent URL dx.doi.org/10.1111/ajt.14280, hdl.handle.net/1765/99704
Journal American Journal of Transplantation
Citation
van den Bosch, T.P.P, Hilbrands, L.B, Kraaijeveld, R, Litjens, N.H.R, Rezaee, F, Nieboer, D, … Rowshani, A.T. (2017). Pretransplant Numbers of CD16+ Monocytes as a Novel Biomarker to Predict Acute Rejection After Kidney Transplantation. American Journal of Transplantation. doi:10.1111/ajt.14280