This article assesses the consequences of the transition to a post-industrial urban economy for labour demand and ethnocentrism in the 22 Dutch urban agglomerations. Using municipal-level data as well as surveys, it is shown that the labour market of the least post-industrial cities yields low labour demand for lower-educated urbanites (upgrading/professionalisation thesis), while the labour market of the most post-industrial cities yields high labour demand for lower-educated urbanites (polarisation thesis). It is furthermore found that lower-educated natives in the former are more ethnocentric than in the latter. However, contrary to what is often claimed in urban studies and the social sciences at large, this proves not to be driven by job scarcity in the least post-industrial cities. The article concludes with suggestions on what might be responsible for this finding.