In this paper we present an FDA validated method to analyze ten antiarrhythmic drugs (atenolol, bisoprolol, carvedilol, diltiazem, flecainide, lidocaine, metoprolol, propranolol, sotalol and verapamil). A simple and fast sample preparation protocol with protein precipitation followed by ultra performance liquid chromatography (UPLC) for chromatographic separation and mass spectrometric detection applying electrospray ionization (ESI+) and selected reaction monitoring mode (MS/MS) was used. Only 50 μl plasma sample is needed for the simultaneous quantification of all compounds within a 5 min run-to-run analysis time. Sotalol-D6, carvedilol-D5 and verapamil-D6 were used as internal standards. The method was validated according to the FDA guidelines. Correlation coefficients were higher than 0.998 for all compounds. Intra- and interday accuracies were within 15 CV(%) for all analytes. The method is currently successfully applied for routine analysis in our hospital.

Additional Metadata
Keywords Antiarrhythmic drugs, Human plasma, LC-MS/MS, Ultra performance liquid chromatography
Persistent URL dx.doi.org/10.1016/j.jchromb.2019.121793, hdl.handle.net/1765/120254
Journal Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Citation
van Domburg, B, van der Nagel, B.C, Andrews, L.M, & Koch, B.C.P. (2019). High-throughput quantification of ten antiarrhythmic drugs in human plasma using UPLC-MS/MS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1129. doi:10.1016/j.jchromb.2019.121793