Purification of biotinylated proteins using single walled carbon nanotube-streptavidin complexes
In this study, Single walled carbon nanotube (SWNT)-streptavidin complexes were used to capture and purify biotinylated proteins, including bio-GFP and bio-DBS using a pull-down method. The purification conditions were systematically studied, including surface blocking of SWNT using chicken egg albumin (CEA), the ratio of SWNT-streptavidin complexes to the cell lysate, as well as the centrifugation speed. Optimization of the protein purification using SWNT-streptavidin complexes shows the possibility of carbon nanotubes as a promising candidate for protein purification applications. The SWNT-streptavidin could be used as a scaffold to analyze protein structure directly by cryo-transmission electron microscopy, which provides better understanding in protein–protein interactions and biological processes.
|Keywords||Biotinylated proteins, Chick egg albumin, SWNT-streptavidin|
|Persistent URL||dx.doi.org/10.1166/jnn.2017.12716, hdl.handle.net/1765/95654|
|Journal||Journal of Nanoscience and Nanotechnology|
Wang, R. (Run), Boleij, M. (Marissa), Yin, Q. (Qu), Galjart, N.J, Lin, B. (Bencai), Yuan, N. (Ningyi), … Abrahams, J.P. (2017). Purification of biotinylated proteins using single walled carbon nanotube-streptavidin complexes. Journal of Nanoscience and Nanotechnology, 17(2), 926–931. doi:10.1166/jnn.2017.12716