2016-09-13
Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function
Publication
Publication
Cell Reports , Volume 16 - Issue 11 p. 2953- 2966
Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomic data with existing mRNA data to derive robust cell-specific expression signatures with more than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1β secretion in response to ATP.
| Additional Metadata | |
|---|---|
| doi.org/10.1016/j.celrep.2016.08.023, hdl.handle.net/1765/97560 | |
| Cell Reports | |
| Organisation | Department of Gastroenterology & Hepatology |
|
Worah, K., Mathan, T. S. M., Vu Manh, T. P., Keerthikumar, S., Schreibelt, G., Tel, J., … Buschow, S. I. (2016). Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function. Cell Reports, 16(11), 2953–2966. doi:10.1016/j.celrep.2016.08.023 |
|
